腫瘤科論文CerbB2在乳腺癌中的表達(dá)意義
【摘要】 目的 探討C erbB2在乳腺癌中的表達(dá)意義。方法 采用免疫組化SP法檢測(cè)161例乳腺浸潤(rùn)性導(dǎo)管癌中CerbB2的表達(dá)。結(jié)果 CerbB2在<50歲與≥50歲年齡組表達(dá)率分別為27.43%和31.25%,差異無統(tǒng)計(jì)學(xué)意義(P>0.05);在浸潤(rùn)性導(dǎo)管癌Ⅰ、Ⅱ、Ⅲ級(jí)中表達(dá)率分別為19.05%、24.21%、42.22%,在Ⅰ、Ⅱ級(jí)中明顯低于Ⅲ級(jí)中(P<0.05);在淋巴結(jié)無轉(zhuǎn)移組、低轉(zhuǎn)移組表達(dá)率分別為20.43%、29.41%,明顯低于高轉(zhuǎn)移組44.0%(P<0.05)。結(jié)論 CerbB2的表達(dá)與乳腺癌的組織學(xué)分級(jí)高,淋巴結(jié)高轉(zhuǎn)移率密切相關(guān)。CerbB2的檢測(cè)可作為乳腺癌預(yù)后判斷的重要參考指標(biāo)。
【關(guān)鍵詞】 乳腺浸潤(rùn)性導(dǎo)管癌;免疫組織化學(xué);CerbB2;癌基因 The expression of CerbB2 in Peast invasive ductal carcinoma WANG Chunyang, HU Jiangong*,SHAO Xuezhai,et al. Department of Pathology, Affiliated Hospital of Chengde Medical college,Hebei,Chengde 067000,China
【Abstract】Objective To investigate the significance of expression of C erbB2 in Peast carcinoma.Methods The expression of CerbB2 was detected by SP immunohistochemical technique in 161 cases of Peast invasive ductal carcinoma.Results The expressing rates of CerbB2 were 27.43% and 31.25% in the age group above 50 and below 50 years respectively, and there was no significant difference between the two age groups(P>0.05). The positive expressing rates of CerbB2 were 19.05%, 24.21% and 42.22% in Peast carcinoma tissues at grade 1, 2 and 3 respectively,which was higher in grade 3 than that in other two groups (P<0.05). CerbB2 expressing rates in patients without and with low frequency of lymph node metastases were 20.43%, 29.41% respectively,which was significantly higher than that (44.0%) in high frequency of lymph node metastases (P<0.05).Conclusion The overexpression of CerbB2 was closely related with a high histological grade and high frequency of lymph node metastases.CerbB2 may be a valuable prognostic indicator for Peast invasive ductal carcinoma.
【Key words】Peast invasive ductal carcinoma; immunohistochemistry; CerbB2; oncogene 乳腺癌是女性最常見的惡性腫瘤之一,在西方國家其發(fā)病率和病死率均居女性惡性腫瘤之首。我國女性乳腺癌的發(fā)病率亦不斷上升,且有年輕化趨勢(shì)。組織學(xué)分級(jí)高、臨床分期高和有淋巴結(jié)轉(zhuǎn)移是公認(rèn)的預(yù)后不良指標(biāo)。近年來隨著分子生物學(xué)的不斷發(fā)展,乳腺癌的分子預(yù)后指標(biāo)越來越受到人們的關(guān)注。CerbB2是一種重要的癌基因,其產(chǎn)物過度表達(dá)與腫瘤惡性程度高、生長(zhǎng)快、預(yù)后差密切相關(guān)[1]。為探討CerbB2在乳腺癌的表達(dá)意義,本研究采用免疫組化方法檢測(cè)乳腺浸潤(rùn)性導(dǎo)管癌組織中CerbB2的表達(dá)醫(yī)學(xué)全.在線m.zxtf.net.cn。
1 資料與方法
1.1 一般資料 標(biāo)本均為我院2005至2006年乳腺浸潤(rùn)性導(dǎo)管癌簡(jiǎn)化根治手術(shù)標(biāo)本;颊呷繛榕,共161例,年齡26~78歲,平均年齡48.6歲;<50歲113例,≥50歲48例;其中Ⅰ級(jí)21例,Ⅱ級(jí)95例,Ⅲ級(jí)45例;無淋巴結(jié)轉(zhuǎn)移93例,淋巴結(jié)轉(zhuǎn)移<3枚17例,≥3枚51例。病理診斷參照WHO《乳腺及女性生殖器官腫瘤病理學(xué)和遺傳學(xué)(2003)》進(jìn)行分類。 1.2 試劑 CerbB2單克降抗體、SP免疫組化染色試劑盒、DAB顯色試劑盒均購自福州邁新生物技術(shù)公司。 1.3 方法 標(biāo)本均經(jīng)10%中性甲醛固定,5 μm連續(xù)切片,每例2片,1片做HE染色復(fù)核病理診斷,另1片檢測(cè)CerbB2的表達(dá)。石蠟切片常規(guī)脫蠟入水;加3%H2O2溶液50 μl阻斷過氧化物酶;加熱修復(fù)抗原20 min;加10%正常山羊血清封閉,室溫孵育10 min,加一抗(1∶50稀釋);4℃過夜;加生物素標(biāo)記的二抗,37℃孵育30 min;加辣根酶標(biāo)記的鏈霉卵白素溶液,37℃孵育30 min;DAB顯色。以上各步驟間均用PBS緩沖液漂洗3次,每次5 min。蘇木素復(fù)染,脫水,透明,封片,鏡下觀察。 1.4 判斷標(biāo)準(zhǔn) 結(jié)果判斷采用美國FDA新判斷標(biāo)準(zhǔn):CerbB2 陽性部位在細(xì)胞膜,細(xì)胞膜出現(xiàn)連續(xù)的棕黃色顆粒,且著色細(xì)胞數(shù)>30%為陽性著色。 1.5 統(tǒng)計(jì)學(xué)分析 計(jì)數(shù)資料采用χ2檢驗(yàn),P<0.05為差異有統(tǒng)計(jì)學(xué)意義。